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KMID : 1138520110140010079
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Journal of Pharmacopuncture
2011 Volume.14 No. 1 p.79 ~ p.86
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The Comparative Study of Anti-inflammatory, Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Cuscutae Semen
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HwangBo Min
Seo Hyung-Sik
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Abstract
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Objective: This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Cuscutae Semen(CS) extracted with two kinds of solvents, ethanol and distilled water.
Methods: Two kinds of CS extractions were prepared 20, 50, 100 ??/??. The cytotoxicity was measured by MTT assay in Raw 264.7 cell. The anti-inflammation effect was measured by inhibitory efficacy of NO Production in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes.
Results: 1. Two kinds(100 ??/??) of CS extraction groups had 50% cytotoxicity in Raw 264.7 cell. 2. All of CS extraction groups were not showed significantly inhibitory effect on NO production. 3. All of CS extracted with ethanol only showed dose-dependently significantly scavenging effect of DPPH radicals. 4. Two kinds of CS extractions did not have a inhibitory effect on Propionibactrium acnes.
Conclusion: Two kinds(100 ??/??) of CS extraction groups have 50% cytotoxicity. Two kinds of CS extractions have not the inhibitory effect on NO production and Propionibactrium acnes. CS groups extracted with ethanol only have a significantly scavenging ability of DPPH radicals. This study suggests that CS extracted with ethanol was effective in anti-oxidation.
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KEYWORD
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Cuscutae Semen, Extration Solvent, Antiinflammatory, Antioxidant, anti-bacterial effect
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